Histograms are concatenated using FlowJo software from one of two independent experiments displaying equal representation from 4–5 individual mice. Black numbers indicate the percentage of P14 CD8 T cells positive for CD25 and grey numbers indicate the gMFI of CD25 + P14 CD8 T cells. Shaded graphs represent isotype control staining and open graphs represent staining on gated 2° effector Thy1.1 P14 CD8 T cells. D) Histograms showing the expression of CD25 on 2° effector P14 CD8 T cells isolated from the spleen 5 days after transfer. Data are presented as mean+ SEM of 3 mice per group. C) Log geometric mean fluorescence intensity (gMFI) of CD25 + 2° effector P14 CD8 T cells. B) Percentage of 2° effector P14 CD8 T cells positive for CD25 on various days after transfer. Histograms are concatenated using FlowJo software from 1 of 2 independent experiments displaying equal representation from 3 individual mice. Shaded graphs represent isotype control staining and open graphs represent specific Ab staining on gated 2° effector Thy1.1 P14 CD8 T cells. The timing of stimulation regulates IL-2 sensitivity and expression of cell-cycle related proteins.Ī) Representative histograms showing the expression of the CD25 molecule on 2° effector Thy1.1 P14 CD8 T cells isolated from the spleens of ‘early’ and ‘late’ groups of mice on indicated days after transfer. The Timing of Stimulation and IL-2 Signaling Regulate Secondary CD8 T Cell Responses Fig 5
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